Induction of DNA Damage Signaling Genes in Benzidine-Treated HepG2 Cells

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Title:	Induction of DNA Damage Signaling Genes in Benzidine-Treated HepG2 Cells
Authors:	Chen,SC;Hseu,YC;Sung,JC;Chen,CH;Chen,LC;Chung,KT
Contributors:	生命科學系
Keywords:	CYCLE ARREST;OXIDATIVE STRESS;HUMAN-LYMPHOCYTES;REPAIR PATHWAY;COMET ASSAY;G2 CELLS;EXPRESSION;MUTAGENICITY;ACTIVATION;4-AMINOBIPHENYL
Date:	2011
Issue Date:	2012-03-27 18:10:51 (UTC+8)
Publisher:	國立中央大學
Abstract:	We examined genotoxicity and DNA damage response in HepG2 cells following exposure to benzidine. Using the Comet assay, we showed that benzidine (50-200 mu M) induces DNA damage in HepG2 cells. DNA damage signaling pathway-based PCR arrays were used to investigate expression changes in genes involved in cell-cycle arrest, apoptosis, and DNA repair and showed upregulation of 23 genes and downregulation of one gene in benzidine-treated cells. Induction of G2/M arrest and apoptosis was confirmed at the protein level. Real-time PCR and Western blots were used to demonstrate the expression of select DNA repair-associated genes from the PCR array. Upregulation of the p53 protein in benzidine-treated cells suggests the induction of the p53 DNA damage signaling pathway. Collectively, DNA damage response genes induced by benzidine indicate recruitment complex molecular machinery involved in DNA repair, cell-cycle arrest, and potentially, activation of the apoptosis. Environ. Mol. Mutagen. 52:664-672, 2011. (C) 2011 Wiley Periodicals, Inc.
Relation:	ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
Appears in Collections:	[Department of Life Science] journal & Dissertation

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