Dioscorea Phytocompounds Enhance Murine Splenocyte Proliferation Ex Vivo and Improve Regeneration of Bone Marrow Cells In Vivo

NCU Institutional Repository > 生醫理工學院 > 生命科學系 > 期刊論文 > Item 987654321/50813

請使用永久網址來引用或連結此文件: http://ir.lib.ncu.edu.tw/handle/987654321/50813

題名:	Dioscorea Phytocompounds Enhance Murine Splenocyte Proliferation Ex Vivo and Improve Regeneration of Bone Marrow Cells In Vivo
作者:	Su,PF;Li,CJ;Hsu,CC;Benson,S;Wang,SY;Aravindaram,K;Chan,SI;Wu,SH;Yang,FL;Huang,WC;Shyur,LF;Yang,NS
貢獻者:	生命科學系
關鍵詞:	TRADITIONAL CHINESE MEDICINE;ECHINACEA-PURPUREA;FED RATS;BACTERIA;MODULATION;DIOSGENIN;EXTRACTS;SPLEEN;MICE;GUT
日期:	2011
上傳時間:	2012-03-27 18:10:35 (UTC+8)
出版者:	國立中央大學
摘要:	Specific cytokines have been tested clinically for immunotherapy of cancers; however, cytotoxicity has often impaired their usefulness. Consequently, alternative approaches are increasingly desirable. Dioscorea spp. tuber is a widely used traditional Chinese medicinal herb claimed to confer immunostimulatory activity. In this study, we evaluated Dioscorea as an adjuvant therapy for use alongside chemotherapy for cancer. Phytocompounds from Dioscorea tubers were ethanol fractioned and used for ex vivo splenocyte proliferation assay or in vivo force-feeding of mice pre-treated with the chemotherapy agent 5-fluorouracil. Co-treatment with a 50-75% ethanol-partitioned fraction of the tuber extract of D. batatas (DsCE-II) and interleukin (IL)-2 resulted in a significantly higher rate of murine splenocyte cell proliferation ex vivo than treatment with DsCE-II or IL-2 alone. This DsCE-II fraction, which contains a polysaccharide with a high proportion of beta-1,4-linkage mannose (>= 64%), also promoted the regeneration of specific progenitor cell populations in damaged bone marrow tissues of 5-fluorouracil-treated mice. Colony-forming unit (CFU) analyses demonstrated that the population of CFU-GM cells, but not CFU-GEMM or BFU-E cells, preferentially recovered to similar to 67% in the bone marrow of immune-suppressed mice fed with DsCE-II. DsCE-II efficacy level was similar to 85% of that obtained by subcutaneous administration of recombinant G-CSF proteins (5 mu g kg(-1)) in mice tested in parallel. This study suggests that the DsCE-II fraction of D. batatas extract may be considered for further development as a dietary supplement for use alongside chemotherapy during cancer treatment.
關聯:	EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
顯示於類別:	[生命科學系] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	645	檢視/開啟

在NCUIR中所有的資料項目都受到原著作權保護.

社群 sharing

資料載入中.....